FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n02_zt2wtlarvs6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n02_zt2wtlarvs6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1320122
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG145731.1039131231810393No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA55490.4203399382784318No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTAT5950.031.3055138
CACTATA6100.029.40474939
TTTCGGA5100.029.0964341
CTATAGG1202.6775524E-928.7581041
GATCTGG26700.024.4208266
ACTATAT8050.023.5637840
ATCTGTG7050.023.4888617
CTATTAG750.001989913623.0064831
ATCTGGC10300.022.776217
TCTAGGG4250.021.9188392
ATTGTAC953.066398E-421.7890073
GACACTA9600.021.5587737
GGGACAC12850.021.20644835
AGTTCCG5050.021.17375232
CGGGATT2451.8189894E-1221.12279715
AGGGACA8500.021.1022334
CGATCTG107100.020.8742945
TGGACTG3000.020.7003425
ATCTTTT22700.020.5171837
CGAGTAT1356.816914E-620.44710526
Produced by FastQC (version 0.11.2)