FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n02_zt2wtlarvs67.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n02_zt2wtlarvs67.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1294742
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG102810.794057812290016No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA71660.5534693398375893No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTGT15350.032.1503869
TTCTATA802.7609185E-630.189022
TTTCGGA5300.029.9541651
ACTGTAC703.851075E-529.5717773
TCTAGGG1651.1459633E-1025.0921752
CGTGTAG5150.024.79436369
TTACGTG19650.024.7550668
CGATCTG71650.024.748815
TTCCGAT194550.023.8169292
TCCGATC199000.023.3701253
CCGATCT201700.023.0050834
ATCTTTT23350.022.7513777
GATCTTT26400.021.821596
GATCTGC24550.021.779816
CTTTTTT22600.021.6747349
ATCTGAG5750.021.5977527
TCTTTTT23750.021.351468
ATCTGGG8750.021.289217
CTTCCGA223300.021.0041921
GATCTGG29150.020.9463586
Produced by FastQC (version 0.11.2)