FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n02_zt2wtlarvs66.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n02_zt2wtlarvs66.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112322
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31162.7741671266537278No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11641.0363063335766813No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2070.18429159024946137No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGAC150.00234705569.0218363
GTGCAAA150.00235120668.99112
GTCCGCA150.00235120668.991144
AGAAACG309.055735E-657.5181967
CTCGGAA252.5588242E-455.1928759
TGTTTAA200.007345595451.7433235
TAATAGC200.007345595451.7433217
AAATCGA200.007345595451.743326
CTCGTTT200.007345595451.743329
CATAGGG200.007345595451.7433227
TCTAAAA200.007345595451.7433236
ACGTCTA408.205607E-751.7433221
TAACTCA200.007345595451.7433249
GACTAAT200.007345595451.7433240
CATGGTT506.877417E-848.29376613
AATTTGG306.291038E-445.99406419
TCCACTC306.291038E-445.99406417
AATCAGG551.4554462E-743.90342336
GCAACGG551.4554462E-743.90342320
GGGTTCG651.12395355E-842.4560641
Produced by FastQC (version 0.11.2)