FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n02_zt2wtlarvs65.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n02_zt2wtlarvs65.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1757782
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG123010.6998023645708057No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22470.12783155135278437No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTGTG7550.028.3302637
CGATCTG98900.025.987585
CACTATA5350.025.79213739
GATCTGG29150.025.5635416
ATCTGGC11450.024.4053697
TCTAGGG4400.024.3060762
GAGGTGT20900.024.27033669
TCTATGC3150.024.0945038
TCTGTCC3600.023.9576038
ACACTAT5850.023.5876838
AGTTCCG15050.022.46251332
ACTATAT5550.022.37578640
ATCTAGC9600.022.280577
CCGATCT284600.022.267314
GACACTA6200.022.25611937
GATCTAG19750.022.1841356
TCCGATC294700.021.6446343
TTCCGAT295950.021.623772
TAAGTTC16200.021.50615530
GTTATCG5150.021.4374611
Produced by FastQC (version 0.11.2)