FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n02_zt2wtlarvs63.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n02_zt2wtlarvs63.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1847110
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG265311.436351922733351No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA54690.2960841530823828No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAGAC2100.032.8604552
TTTCGGA4150.029.1056291
ACGGTGT16200.024.92660769
GTCTAGG2250.023.0073031
TCTAGGG4300.022.4673822
AGACATA7150.021.711365
GCATTAT800.002895662321.5681859
ATCTGGC10500.021.0267077
GCTAAGC1154.605801E-520.99927563
TAGCCCG1657.93043E-820.90723610
TATACTA2402.910383E-1120.1232138
ACACTAT12450.019.95003738
TTAACTA1056.01421E-419.71947955
ATCTTTT20000.019.4907727
GACACTA13150.019.41272737
CACTATA11200.019.4045339
GTATTGG2153.1377567E-919.2619291
GTATCAT2153.1559466E-919.25358436
GACATAA7050.019.0834146
ATCTGTG7250.019.0327957
Produced by FastQC (version 0.11.2)