FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n02_zt2wtlarvs62.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n02_zt2wtlarvs62.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1408367
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG199751.4183092901211118No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA42390.3009868876507331No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGGA4750.031.2421021
ATCTGCG10100.028.0083877
CGATCTG83300.027.500095
GATCTTG10200.026.3809286
GATCTGG27900.026.2136366
TCTGCGC6500.024.9448058
CACTATA2500.024.8404239
ATCTGGG9450.024.0939377
ACACTAT2900.023.79350738
CCGATCT238200.023.3906234
TTCCGAT240450.023.2611392
CTAGGGG3750.023.0003913
TCCGATC245200.022.7658843
GATCTGC34700.022.269696
TAGGTGT16800.022.1860469
ATCTGGC11200.022.1773747
TCTGACC4900.021.1213078
ATCTGAG5600.020.9452977
CTTCCGA269850.020.8847431
ATCTGTG6450.020.8593397
Produced by FastQC (version 0.11.2)