FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n02_zt2wtlarvs61.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n02_zt2wtlarvs61.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1713872
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG204391.1925628051569779No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA43730.2551532436494674No Hit
GTCCTGGAGACAGTGGGGAAGTCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTACCT19110.11150190912740275No Hit
GTATAGGGCCTGACGCCTGCCCGGTGCCTGAAGGTTAAGTGGAGGGGTGCAAGCTCTGAAATGAAGCCCAGGTAA18700.10910966513251864No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAATGGCGTAACGACTTCCCCACTGTCTC17430.10169954348982888No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGGA4700.030.107311
ATCTGGC10500.027.597717
ATCTGTG7250.026.6460677
ATCTAGC6850.024.6767867
CTTGTGT19350.024.61266969
ATCTGAG7050.023.4874157
CACTATA3850.023.2967739
CGATCTG127350.021.0206385
TCTGTGC5450.020.8881768
GATCTGC37050.020.5772426
CCGATCT316400.020.2578014
TCTGAGC4800.020.1233318
TTCCGAT320350.020.0869162
TTGTGTA12600.019.99460869
GATCTAG17850.019.9059126
TCCGATC327150.019.7315253
TCTGACC6200.019.4741928
ATCTGCG9300.019.2887217
TCTAGGG3400.019.281192
GATCTGA34000.019.1763526
Produced by FastQC (version 0.11.2)