FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n02_zt2wtlarvs60.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n02_zt2wtlarvs60.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1741603
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG209131.2007903064016312No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA43560.25011440609599317No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGGA8050.028.292961
GATCTGG44600.025.5268426
CGATCTG140150.024.3948735
ATCTGCG12350.024.0242317
ATCTGGC17150.023.9387157
AGTGTGT26350.023.1818469
TGTGTAG15500.022.04239369
TCTGAGC7250.021.413748
ATCTAGC9350.021.4010167
ACACTAT6400.021.02338638
GACACTA6750.020.95550337
TCTAGGC4000.020.6999478
ATCTGGT10850.020.6681527
ATCTGGG17550.020.4443937
ATCTGTG8950.020.4301177
TCTGCGC7100.020.40848
TAGACAG4400.020.3863125
ATCTAGG6450.020.3255317
GATCTTG19200.020.3046366
CACTATA6000.020.1249539
Produced by FastQC (version 0.11.2)