FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n02_zt2wtlarvs57.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n02_zt2wtlarvs57.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1391277
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG181371.3036224993297525No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20250.14554973596199752No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTGG28250.024.3005546
TACACCG2300.023.9987875
CGATCTG95600.023.9250455
GACACTA5150.022.77554737
ATCTGTG6550.022.6468947
ATCTGGC10150.022.4315577
TCTGACC4800.022.2793248
TTTCGGA5300.022.1397131
CGGGAAC4750.021.78758815
ACTATAT4550.021.22740740
ACACTAT5050.021.17714938
TCTGGCC6700.021.1101158
CACTATA5250.021.0275139
GATCTTT19900.020.975576
GATCTGC35800.020.9102026
TACGTCT1657.92661E-820.9072824
GAGGTGT12900.020.86616369
TCTGGTC4300.020.858668
ATCTTTT15400.020.8326137
CCGATCT281300.020.295974
Produced by FastQC (version 0.11.2)