FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n02_zt2wtlarvs54.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n02_zt2wtlarvs54.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences968067
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG67410.6963361007037736No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14840.1532951748174455No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTAT1900.030.86992338
CACTATA2000.025.87625939
CGATCTG50800.023.4931055
CCGATCT138800.021.8437614
TCCGATC142250.021.4142953
TTCCGAT142300.021.3593882
GCACTGA1304.929434E-621.2263186
GATCTGG18800.021.0992986
ATCTAGC5600.020.9420367
CTATATA2800.020.94095441
ATAGTCG1657.930612E-820.9047058
TAGGTGT10350.020.67622269
CAGTACT1206.396056E-520.1207794
CTTCCGA152300.020.0506531
GTATGTA2453.8198777E-1119.7172831
CGATCTA31200.019.2363515
GATCTAG11000.018.8142366
GATCTGC20850.018.6939226
ATCTGGC7600.018.6079397
CGATCTT26350.018.1954255
Produced by FastQC (version 0.11.2)