FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n02_zt2wtlarvs53.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n02_zt2wtlarvs53.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2259835
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG185140.8192633532979178No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA68810.30449125710505415No Hit
CCCTTGGGAGATCGGAAGAGCACACGTCTCCCTTGGGAGATCGGAAGAGCACACGTCTCCCTTGGGAGATCGGAA22650.10022855650965669Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGGA4900.035.9169431
ATTGTAC500.007737272427.5994173
TGTATAC5200.026.53320164
ACTGTTG3750.023.92743769
CTTGTGT20150.023.12132869
GTATAGT2650.022.1374871
ACACTAT10200.021.98385438
ATTGTAT4050.021.29207662
CACTATA10050.021.28218839
ATTACTC2950.021.04667544
TCTGCGT2301.4551915E-1120.999098
GTATATC7250.020.93656237
TCTGAGT1657.928611E-820.9081868
CTGCGTT1004.3378572E-420.6991049
TTGTATA6550.020.53791263
AGGACTA5050.020.4946165
GACACTA12650.020.45320737
GTCTTAC4250.020.299081
GGTAACG4300.020.05860326
ATCTTTT17850.019.90493812
Produced by FastQC (version 0.11.2)