FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n02_zt2wtlarvs52.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n02_zt2wtlarvs52.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2243169
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG185200.8256176864070428No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41170.18353499000744036No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG134650.027.8505825
GATCTGG42150.025.6176036
CCGATCT353000.024.7658184
TTCCGAT360450.024.3980582
ATCTGTG11750.024.0750797
ATCTGGG18250.023.8176787
TCCGATC374350.023.557173
ACTGTAC7050.023.49053
GATCTGC53250.023.1929046
CTTCCGA384600.023.0616261
CACTATA4400.021.95267139
ATCTGCG15050.021.7760377
GATCTTG18650.021.4570926
CGATCTT69300.021.4065325
CGATCTA73800.021.176445
TCTGAGC5600.020.9451088
TCTGTGC8450.020.8211738
CGATCTC97450.020.6748375
ACACTAT5300.020.17752538
AGTGTGT24850.019.72095969
Produced by FastQC (version 0.11.2)