FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n02_zt2wtlarvs50.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n02_zt2wtlarvs50.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1663746
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG73270.44039174248953866No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39230.23579320401070836No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGTTG3800.036.32956369
ACACTAT14450.033.66295238
CACTATA14200.033.52676439
ACTATAT16500.030.73415240
CTATATA19150.029.18240441
GACACTA19650.028.44155937
TCTAGGG2950.028.0674672
ATGCCAA5800.027.95572962
GGGACAC27450.027.9004735
ACAAAAT30150.026.55014452
GGACACT23500.025.25000836
AGGGACA26700.024.9371634
ATATGCC4300.024.87928260
TATGCCA5400.024.28479661
TACTGTT5750.024.00188468
TAGGGAC12000.023.86078533
AGCAGTC4850.023.47324638
ACTCTCC8550.023.41097655
ATACTGT8550.023.40534267
GGATACT12850.023.3598165
Produced by FastQC (version 0.11.2)