FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n02_zt2wtlarvs49.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n02_zt2wtlarvs49.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110
Sequences flagged as poor quality0
Sequence length75
%GC31

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCCGATCTTAATATTTTTTATTTTTTTTTTTTTTTTATCTAGGTGAACACCGAAACAAAAAAAAAAAAAATTT10.9090909090909091No Hit
AATCATAACTTATATATAATTTATATTATTTTTTATAGTTGTCAGGCACAGTCTATTATAAAAATCTTAAGCAAA10.9090909090909091No Hit
GTGCACATACGATAGATTTTATATTAATATTTATTATATATTTAATAATTTATTTTATAATTGTTTATATAATTT10.9090909090909091No Hit
GTCCTCTTCCGATCGATTTTTATTTTTAATTTTAATATATTTTTTATATAAATTTAGAAAATAAAAAACACAACA10.9090909090909091No Hit
ATCAATTATATTTTTTTATTATATTTTTTAATTTTTAGTTATATTTATATTTAATTATTTATATATTTTAATTTT10.9090909090909091No Hit
GAGTGGGGGGGTTGGGGGGTGAGGGGGGGTGGGTGGGTAGGGAGACAGGCAGGGGGTAAGGGTGGAGGGGAGGAT10.9090909090909091No Hit
ATTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTAATATATAAAAAAAAAAAAAG10.9090909090909091No Hit
CAGTTAAAAAGCTCGTAGTTGGACCTTGGACCTGGACAGGCGGTCCGCCTCACGGCGAGTACTGTCTTGCTGCTC10.9090909090909091No Hit
ATTTTATTATGATATATATTATATGTTTTTTTATGTTTTTTTAAAAATTATAAAATTTAATATGTTAATTTTTAT10.9090909090909091No Hit
TTTTTGTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTTTTTTTTTTTT10.9090909090909091No Hit
TCCATGGACATTGCCTCCTCATCTCCCATCTCCTTCTCCTTAAGCTGTTCTAAGAACCTCTCCTTTTGGTCCTCT10.9090909090909091No Hit
GTATTTAAAATGTGGTTAAGTTACTAAGGGAACACGGTGGATGACTTGGCGCTAGGAGCCGAAGAAGGACGCAGC10.9090909090909091No Hit
CATCTGGCGCTGCCGGCGAGGGAGAGAGGGGAGATGGCGGTGGGGGGCGGATCAGTAAAAAAAGTGGGGGGGGGG10.9090909090909091No Hit
CACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCACTTTACAAAGAACCGCACTCCTCGCCACACGG10.9090909090909091No Hit
CTTCTGAGGGAGCCGCGGAGGGGGGGGGGGTTGGGCTGGGGGGGCGCGGGGGCAGTGTGGGGGAGGGGGGGGGGG10.9090909090909091No Hit
CTCACGCGCCACGCGACCGTCGCTTCACGCCGCGCGCTTCCAACGCATCGAGCGCCTTTCGTTGCCGTTGGCCAT10.9090909090909091No Hit
GTGCTATTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTATTAATATAAAAAAAATAAG10.9090909090909091No Hit
GTGGAGCACGATCCGCTTGAAATTTGGGAGACGCAGATCGGCGTGGCCCGGGAGGTACTGTACCAAACCGGCGTC10.9090909090909091No Hit
AAAAAACGTAATACTTATTCTCTACCTATCTAAAACTGTAGAAGATGTCTCTTAAAATCAAAAAAAAATGCCGAG10.9090909090909091No Hit
GTGATAATACATGCGTTTTGATGATGTCTATCTGTATAGATCGGAATAGAAAAAGTGTAGTTTATAATAAATATA10.9090909090909091No Hit
CTTCCGATCTGAAGCCGAAGCGCTCCTGGAAGGGCTCGCCGAAGAGGGTGGCGGTGAGGCGGTACCCGCCCTCTT10.9090909090909091No Hit
GTGCTCATCCCATCAATTAATAATTTAAATTAATATATTTTATTATAAAGTACAACAAAACAAAAAAAAGAGGTC10.9090909090909091No Hit
ATAACACTGACACAAACATACAAAGACATGTTATATCTTTAAATTAAACATCCAAAACATCAAATAACTTCCAAG10.9090909090909091No Hit
TTTTTGTTGTTTGAAATATTGATAATAATATAAATAAATTAAAAATATATATTTATTATAAAAAAATATTTATTT10.9090909090909091No Hit
CCATAAGGTTCATGTTTTAATTGCATGTATTAGATCTAGAATTACCACAGTTATACAAGTAACTGTTAACGATCT10.9090909090909091No Hit
CTACATATGTTATTTTTTTTTTTATATTTTTTTATTATAATTTTTTGTATTAAAAAATAATGGAGATGCCAATGT10.9090909090909091No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10.9090909090909091No Hit
AGTCCGAGCTTTTTATAATGTTATTTTTATGAAAATATAAAGAAAATTGGCAGAGGAGGCGAGGAGGAGGGACCT10.9090909090909091No Hit
GTTCGGGTCGTTTTTTTTTTTTGTATTTTTTTTTTTTTTTTTTTTTTTGTTATTTTTTTTTTTTTTTTTATTTTT10.9090909090909091No Hit
TTTCACTACAGATATTATATTTATATTTTTTTATTTTTTTTTTTACAGAAACACAAAAACAATAGTTTAAGATAA10.9090909090909091No Hit
CTCTTCAACACGTTGTAAATAAATTTGAATTTAAGGGTAGCAAACAACCGAAGAGATAACCGCAGAAAGCATAAA10.9090909090909091No Hit
CCAGTGGGAGATATTAATTCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.9090909090909091No Hit
GAGCAGAACCGATCTTAAAAATTTATATATTTTATATTTATTTTTTTTTTTATTTTTATTTATTTTAAAATAAAT10.9090909090909091No Hit
TTGCAATTCCGATCTTATAAATTTTTATGGTTTTATTGTTTTATATGCCAAAGGTTGAGCGACACCGCAAGCCGC10.9090909090909091No Hit
GAAATAACGAAAAAATAAAAAAAAATTTTTATTTTTTTTTTATTTAAGAGGTCAACCAGATGATGTTTTTTTATA10.9090909090909091No Hit
ATGTTTGTTGGTGTTTTTGATATTGAGTTATTTAAGGGTAAAAGGGTGAAAAACTTTAATACATGGGATGACTCT10.9090909090909091No Hit
ACCGAACTTTTTTTTTTTTTTTTTTTTTTATTTTATATTTTTTTTTTTTTATATATATATTTTAAATTTTATATT10.9090909090909091No Hit
GAACATCCCATGTGTGTATATATTTTTTTTTTTTTTAAATTTTTATTATATTAAATTTTTTATAATAAATATTTT10.9090909090909091No Hit
GTATTCAGCCGTCAGAGGTGAAATTCTTGGACCGGCTGAAGACTAACTACTGCGAAAGCATTTGCCAAGGACGTT10.9090909090909091No Hit
CTTTTATTTTTTTATTTTTTTTTTTTATTTTTTTTTTAATTATATTAATATTAATAATATATAAAAAATGTAAAT10.9090909090909091No Hit
CTTCGGATCTCGGCTGGGATGACGTGTGCTGTTAAGATCTGTTTTTTAATTTTTTTAATAATTATATTATAATGG10.9090909090909091No Hit
GAAGACTCGGTAGCTGGGCTGTAGTCTCCTCCTGAAACCGCGAACCGGGGGGAATACTGTCTCTTATACACATCT10.9090909090909091No Hit
CTTAAGATCTTTATATTTTTTTTTTTTTTTAATTTTATAAAAACACAAACAATTCTATTAATAAAATTATCACGG10.9090909090909091No Hit
CTTCATATATTAATATAAAAAAAATATTTATTTTATATTTCTATAATACACAACAGACACTGAAGACGAATAAAA10.9090909090909091No Hit
GAGCTATTACGAACTATTATATATTTTATTTTTTTTTTTTTTTTATTTTATAATTTTTATAAAAATATAAAGAAA10.9090909090909091No Hit
CAAAAAAACATTCACAAAAAATAATCTTAATACTGACCAAAAAATTCACCTCTCCCTACGAAAAACAAAAAAACA10.9090909090909091No Hit
GGCCTGGACTGGAAGGCGGCGCCCCCCTCGCCCCGCCGCGCGCGCTCCTTCAGCACTGACGCTGCCGCGTTAGTG10.9090909090909091No Hit
ACTTGTGATATAAATTTTGAAACTTTTAGTGAGTAGGAAGGTACAATGGTATGCGTAGAAGTGTTTGGCGTAAGC10.9090909090909091No Hit
TTTTTGGTTTTTTTTTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTTTTTTTTT10.9090909090909091No Hit
GCCCTGGGAGACGTGTGCTCTTCCGATCATCACTGTGATACGAATCATAATCAGAACAGCAATAGTAAACGTGTA10.9090909090909091No Hit
ATGCTCTTGAGATCTTATTAATGAATTTATAATTATTATAATAGTTAATTTATATGCTAATTGAGATCGCAAGAC10.9090909090909091No Hit
AAAATTATTATTTATTAATATTTATATTTTTTTTAAAAATAAAATATATTTTATATAATAAAGAAAAATAAAAAT10.9090909090909091No Hit
TCTGAAAACCAATGAAAGCAGAACAGAGGTCTTATTCTGTCTCTTATACACATCTGACGCTGCCGACGAGTAGAG10.9090909090909091No Hit
GTGGGGGGCGGGGGGGGCGGGCGCGGGGGGGGGGGGGGGAGGTCGCGGGGGGGGGGGGGGGGGGCCCGAGGCGGG10.9090909090909091No Hit
CATCGGATAAGTTTGGTATTTATAGATCTAATTAATTAAATTTATTAATGAAATTATGTATATTATAAAAATATT10.9090909090909091No Hit
CTATTTTTTTTAATTTTTATATTTGTTTATTAATTTTTTTTTTTTTTTTTTAATTTTTAATATTAATTATATTTT10.9090909090909091No Hit
CTTTTTTTTTTTATTTTTTTTTTTTTTTATTTTTTTTTTTTTTAATATATTAATAAATAAATATAAAAAGAAAAA10.9090909090909091No Hit
AAATCGAACAAATTAGATTAAATTTGAATTAAAGAATATATATTTTGAAAATAAAATCTCAAAACTAAGAATTAA10.9090909090909091No Hit
GTTCGGGGGGGGGCGAAGGGTGGGGGGGGGGGGAGGGAGGAGGGGGAGGGGGGGGGTAGGAGGGGGAGGGGGGGG10.9090909090909091No Hit
GTTCAGATGTATTTTTAATTTATTTTTTTTTAATATTTTTAAAAAAAATCTAAAAAAAAAAAAAAAAATAATAAG10.9090909090909091No Hit
TCAAAGGGCATCACACCCCTGTAATAGCACAAACTCATTATAGCTATACTCAATATCTCCAAATAAGCCGCAAGG10.9090909090909091No Hit
ATTCCGATCATTCTGATATTAATATATATTTTTTTAGTAGTAAACAAATCAGATCTATATTAAAACAGACTCTAA10.9090909090909091No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.9090909090909091No Hit
CGTGAGAACTTATTTTATTATTTTTTGTTTTTTTTTATTAGTAGCAAGGACAGCGGGCAAGTAATAAGAAACGTT10.9090909090909091No Hit
GATCACACGTCTACCAGCCGAGATCTGAAAAACACAACTCACCCAGCCGACATATAAAAATCAAAACAAAACCAT10.9090909090909091No Hit
TACCTATCAAGAGAAAAAGAAAACACTTCTCAAAAAAACATTAACAAAGAACCAAAACGATGCCAACTCATTAGC10.9090909090909091No Hit
ATTCCGAACATATAATATTTATTTTTTTGTTTTTATTATTATTTTTTGTTTTATTTATAATTAATTTTATTAAAT10.9090909090909091No Hit
GGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10.9090909090909091No Hit
GTTATATTAAGATCTAATTTTTTTGTTTTTATTTATTTTTTTTATTTTTATATATTAATTTTTATTTTTTTATTA10.9090909090909091No Hit
CTTCCGATCTAGGCGTGGAGCCGTGTGCACTTACGATCTAGCCAGGTCTAGTAATGAAATAACTCGCTAGGCGCG10.9090909090909091No Hit
TTTATGGTTAGAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTAACTTTCGTTCTTGATTAATGAAAACATCT10.9090909090909091No Hit
GTGCTATTCCGATCTTTATGTTTTTTTATATTTAAATTTTTAATAGCAAGAAGGGGGTAGTAAAAAGAACCACGT10.9090909090909091No Hit
CCTGGGGATGGAGTAATCTTCAGGTGCGGATGCCGATTTAGAAAAGCGACTGGAAAGGTAGTTGGGAAGTGTGTT10.9090909090909091No Hit
CACCTCTACCTGTGTTTATATTTATTTATATATTTTTATTTTTTTGAAATGAAAAGAAAACGAATAAAATAAAAA10.9090909090909091No Hit
GTGAAACAATATGCCGAAGCCAAAAATTATCCGTGACTGGTTGGCGGCAATGGTCACCGGGTCATTGATCGCAGC10.9090909090909091No Hit
AAGCAGATTCCGACCAATAATATAAAGACGCTAGAGGTGAAATACTTATACATTAATAAAAATAACTTAAGCGAA10.9090909090909091No Hit
GTGATCATAAGATCTATTATTTTTTTATTTTTTTTTTTTATAATTTATAATAAAGATTGAATATAAATATGTATA10.9090909090909091No Hit
ATTCAGATCATTTTTTTTTTTTTTTTTTTTTTTTTATTTTTAGAAAAGATAAAGGAAAAATGGAGAAGTAGAGTT10.9090909090909091No Hit
CATGCGAACTATTTAATTTTTATAATTTAAATTTTTTATGGTAATCCACACAGAAAAGCTAAAAATAATAACAAT10.9090909090909091No Hit
ATCTAGAGTTCAACCAATATAACGATCTTGCGATCGCTTGGTTTTAGCCTAATAAAAGCACATGTCTCATAAGGT10.9090909090909091No Hit
TTGCTATTCCGAAAAATTTATATTTTATATTTTTTTTTAATTTTTTATATTTATTAATAATTATTAAATATAATT10.9090909090909091No Hit
TTACCAGATCGCTTCACAGGCTTACGGAACGCTCCGCTACCGCGTGGCTTACGCCACACCCTAAGCTTCGGTGCA10.9090909090909091No Hit
CAAATAAGAACTTATTTATAATAAATTTTTAAATATATTTATTTATTTTAATTATTATATAATAATTATTTTATT10.9090909090909091No Hit
CTTCCGATCTGAGCGGGGGACGTGTGCTCTTCCGAGCTTTTTTTTTTTTTTTTTGTTTTTTTTTTTTTAAAGAGA10.9090909090909091No Hit
CAAAAGGGGTGCTCTTCCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAA10.9090909090909091No Hit
GTTTAGTGTAATGTGATTTGTTTTTTTTGTGTTTATTTTTTTTAAATTAATATATTTATTTATATTATTAAAATT10.9090909090909091No Hit
GTAGAAGTTTTTCCGCCATTTTTCCAACAATTGATTGGTGATCACACTGGTCTCAGCGAACCTGTGCGTCGAGTT10.9090909090909091No Hit
GACGTGTGCTCTTCCGATCTCCCAAGGGAGATCGGAAGAGCACCTGTCTCTTATACACATCTGACGCTGCCGACG10.9090909090909091No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGAGG10.9090909090909091No Hit
AAAATAATATATAATATTACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.9090909090909091No Hit
AAATATTATTTTGTTATTAATTTTATTTTGATCTTCCGACCCCCACACACTAATACACATATGACGATACACAAC10.9090909090909091No Hit
GGCATCACAGACCTGTTATTGCCTCAAACTTCCATCGGCTTGAGCCGATAGTCCCTCTAAGAAGCAGGCGACCCG10.9090909090909091No Hit
GTGCTCGTCCGGTCTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATATTATTAA10.9090909090909091No Hit
TCAAAAACACAACTGCATAAAGACGCTAAAAGCGCGCTGAAGATCACAGATGTCGCAATAAAATATAAAAAAAAC10.9090909090909091No Hit
GTCGATCGGATAGTGGAAGCCTCTGGAAATAGGCGCCATAGAGGGTGATAGCCCCTTACACGAATCTGAACGGTG10.9090909090909091No Hit
CACTTACTATCTCAACCAGGAGACATGTGCACTTCCAATATCAACCTGTCTCTTATACACATCTGACGATGCCGA10.9090909090909091No Hit
GGCCATCGCTCCACGGATAAAAGGTACGACGGGGAAAACAGGCTGATAACCCCCAAGAGTAATAACGCCGGGGTT10.9090909090909091No Hit
GTGCAGTTCGGTTCTCAAAGGGGATCTTTTTTTTTTTTATATATTTATATTTTATTTTAAATTAAATTATATTAT10.9090909090909091No Hit
GGGGGGTTGGGGGCGGGGGGGGAGGTTAGGGGAGGCAGGAGGGGGTGGGGGGGGGGGTGTGTGGGAGAGGCGGAG10.9090909090909091No Hit
TATCGACGGGGTCGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGGGCTGGAGAAGGTCCCAAGGGTTCGGC10.9090909090909091No Hit
AAGAGAGACGTGTGCTTTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.9090909090909091No Hit
ATACCCAAAAGACGATGAAGACACCAAAAATAAAAATACACTCCTAATAACCCATAACCTTTAAATAAAATGAGT10.9090909090909091No Hit
ATTCAGATCATTAATATAATTATTATTTTAGTTTTTTAAATTTTTAATTATTTATATTATAAAAATATTAAAAAG10.9090909090909091No Hit
ATAAAGAAATACTATTCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.9090909090909091No Hit
TACTTGTTCTCTTACGATCTGTGTTTAAATTAGGATTATGGGAAATTTATGTTATTGTAATTGGAATGAGTAAAA10.9090909090909091No Hit
GGTGGTGGTGCATGGCCGTTCTTAGTTCGTGGAGTGATTTGTCTGGTTAATTCCGATAACGAACGAGACTCAAAT10.9090909090909091No Hit
CTGCAGATATTTGTAGTGTAGTGTGGTTTTTGTTTTGAAGAATTAATTATTATTTTTTTATTTAAATATTGATAT10.9090909090909091No Hit
GTGCTAAAAAGATGTATTATTTTATATTTTTTTATTTTTTATATTACAAAGAGGGAGACTGAGAAAAGGATAAAA10.9090909090909091No Hit
GCTTCATACGGGTAGTACAAATTAAAATTGTGGTTAGTACTATACCTTTATGTATGTAAGCGTATTACCGGTGGA10.9090909090909091No Hit
CTTGACGAAGGGTTAGGCTCTCAGGTTATTTATGGACAAAGGAAGTGCAATATACGAACTGGCTCGGAGAGACAT10.9090909090909091No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTATA150.046.046
TTTTTTT700.09.85714336