FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n02_zt2wtlarvs47.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n02_zt2wtlarvs47.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1581929
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG261141.6507694087408473No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36910.23332273445900542No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTATA6450.028.88323239
TTAATAC1900.027.236383
ACACTAT7450.026.85860438
TTTCGGA5750.025.8069081
ATCTGCG9950.024.2686427
CTATATA10000.023.4551541
ACTATAT8800.023.12736540
GACACTA9000.022.23295637
GATCTGG37650.021.8063266
GACTAGG1906.730261E-1021.7953071
CGATCTG115750.021.5768535
ACTGTTG2800.020.952769
ATCTTTT19950.020.9224747
TCTGCGC6200.020.5864098
GACTCTA2352.0008883E-1120.55284337
ACGGTGT16000.020.49050769
GATCTTT24550.020.233996
TCTAGGG5700.019.974612
GGGACAC12600.019.71395135
ATCTGTG8700.019.4288627
Produced by FastQC (version 0.11.2)