FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n02_zt2wtlarvs46.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n02_zt2wtlarvs46.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1796225
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG86090.4792829406115603No Hit
ATTGAAGACTAACTACTGCGAAAGCATTTGCCAAGGATGTTTTCATTGATAAGGAACGAAAGTTAGGGGATCGAA18380.10232571086584365No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTGT11250.033.44077369
ATCTGGG7350.032.384287
CGATCTG59450.030.0590045
CCGATCT142350.029.833024
GCGCTAT6400.029.6460811
ATCTAGT2500.028.9768877
CGGTATA3100.028.935629
CGATCTA30450.028.550255
GCTATCG6550.028.4381113
TGCGCTA6600.028.22502910
TTCCGAT154150.027.8688642
AGTTTCG3500.027.60241722
CGCTATC7050.027.39902512
GGTATAA3300.027.17738730
GGTCCAA2800.027.10498811
CGATCTT25450.026.568375
TCCGATC162950.026.3178063
GATCTGG24850.026.0978016
TTAGGTG14550.026.08403468
CTCTACT6750.026.0762121
Produced by FastQC (version 0.11.2)