FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n02_zt2wtlarvs40.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n02_zt2wtlarvs40.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences660305
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG259833.9349997349709604No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15670.2373145743255011No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTAT3150.031.76190438
TCCTACA553.2540888E-431.3660132
TTTCGGA2100.031.2190171
TCATTAG802.75604E-630.19207429
GACGTTA703.8485545E-529.57142839
GTCTAGG1650.029.2771661
TGCGTAA1005.0056224E-727.60418551
CTAGTCC500.0077325727.614
ACTATAT3500.026.6062240
TCTGAGC2150.025.6763658
CACTATA3650.025.52054839
TAGACTC951.0480329E-525.42683461
GACACTA4650.025.22580537
GAATATG700.001332067324.6465931
AGTAGAC700.001335011924.63725766
ATCTGTG2900.023.7949097
GGTAAGT2500.023.46355628
GTATGAA750.001990372823.0034871
CTCCTAG1052.2762057E-523.0034851
TAGTCCA902.1250207E-423.017
Produced by FastQC (version 0.11.2)