FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n02_zt2wtlarvs3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n02_zt2wtlarvs3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2051307
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA132030.6436384217476955No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG93250.45458822107076124No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTATA14150.032.67073439
ACACTAT14550.031.77256638
TTTCGGA6650.031.6583421
GACACTA16650.026.93640537
CTATATA17350.025.64578441
ACTATAT15200.025.64376640
CGTGTAG15600.025.2196569
GGACACT19150.023.96036336
ACTGTTG2900.022.6107269
GGGACAC25100.022.54138635
GTATTGG2300.022.5083371
AGGGACA14850.022.07026934
TGCCAAA11650.019.83935763
CTGGGGT5800.019.6274874
ATCTTTT32400.018.9514797
AACTGTA3500.018.72728765
GGACAGA13450.018.7227126
CAAAATA28950.018.7126753
GAACGTA2406.0936145E-1018.68758618
TAGGGAG45700.018.5693914
Produced by FastQC (version 0.11.2)