FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n02_zt2wtlarvs39.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n02_zt2wtlarvs39.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences949581
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34780.36626680609658363No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTGAG2800.032.0351527
CGATCTG38100.031.4207085
GATCTTG5850.030.6661286
CCGATCT97550.028.7509274
TTCCGAT98000.028.5882242
TCCGATC99800.028.2079223
GCACAGC500.00773093827.60242528
CTTCCGA103900.027.1972871
GATCTAG8550.026.227616
CGATCTA21550.026.0946715
ACTGTGC2800.025.8745468
TCTGAGC2700.025.5551078
ATCTGGT3600.024.916237
TCTAGGC1401.3875251E-824.6424268
CGATCTT15550.024.4047155
CTAGGCC851.4432134E-424.3525129
GATCTGC14050.024.3091836
ATCTAGC4550.024.263317
CGACAGT1301.8451101E-723.88671329
GATCTCG7800.023.8841956
Produced by FastQC (version 0.11.2)