FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n02_zt2wtlarvs38.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n02_zt2wtlarvs38.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences658043
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG47580.7230530527640291No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19240.29238210876796805No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG553.25359E-431.3668161
AGTAACC756.1585015E-527.600759
ACTGTTG1155.6259523E-827.00273769
CACTATA2300.025.50064739
ACACTAT2300.025.50064738
CGGGAAT700.001332452924.64535552
GATCTGG14500.023.078146
GACACTA3450.023.00058437
ATCTGGC6100.022.6218057
GGGACAC4900.022.53118335
TAGGTGT6350.022.27784769
GGAACGT1403.7697282E-722.17913417
CGATCTG50950.021.8026245
AGGGACA3350.021.62741534
TTTCGGA1605.7028956E-821.5646861
ACTTGCG800.002897701221.56304721
TTTGTAC800.002897701221.5630474
CTGACTA800.002897701221.56304728
AAGTAAC1304.9154532E-621.23130858
CTAGGGA3750.021.1605363
Produced by FastQC (version 0.11.2)