FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n02_zt2wtlarvs37.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n02_zt2wtlarvs37.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1807515
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG143180.7921372713366142No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA64400.3562902659175719No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTAT8450.028.98593538
CACTATA9550.026.00846939
CTTGTGT16200.025.77651869
TTTCGGA5550.024.8698311
ACTATAT10850.023.8427940
GATCTGG41150.022.7194146
ATCTGTG9800.021.8254557
CTATATA13300.021.78478241
CGATCTG140750.021.6181165
GATCTTG16200.021.0822946
ATCTGGG14600.021.0297857
GACACTA12800.021.02181837
GTTAACG1154.6052723E-520.99954665
GGGACAC18700.020.47703435
ATCTGGC16350.020.466887
TCTAGGG6050.019.9610062
AGGGACA13800.019.7484834
TGGGACA8800.019.60076134
ATCTGAG9550.019.506897
ACATAAT10550.019.29983169
Produced by FastQC (version 0.11.2)