FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n02_zt2wtlarvs35.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n02_zt2wtlarvs35.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2153423
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG162790.7559592332765092No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50100.2326528508333012No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTATA8500.031.25115239
ACACTAT9450.029.20467838
GACACTA10300.029.13910937
ACTATAT8450.028.98381440
TACGTGT21950.028.45769569
TTTCGGA5400.028.1199511
TCTGTAC26550.028.06814827
CTGTACT25700.025.37191228
ATCTGTA32450.024.98490326
TGTACTG26700.024.93851529
TTACGTG25250.024.45886668
CTATATA10100.024.24827241
GATCTGG43650.022.9986846
ACAAAAT17050.022.86910452
GGACACT13750.022.8314236
GTACTGG37800.022.642041
CTAGACT1554.09309E-822.2567924
CGTGTAG9650.022.17277569
TGGGACA11650.021.91290934
GGGACAC19300.021.62829635
Produced by FastQC (version 0.11.2)