FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n02_zt2wtlarvs32.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n02_zt2wtlarvs32.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2046897
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG974174.759252663910299No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA54990.26865054763380863No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGGA6700.034.504621
TCTAGCC4450.025.5833198
CTACTAG1551.5243131E-924.4871481
ATAGTAT2500.023.4562662
GTGTAAC902.1248621E-423.00364351
ATCTAGC9800.022.8818027
ATCTGTG8950.022.742177
CACTATA6400.021.5611739
AGAGTGT29350.020.69506369
ATCTTTT27750.020.512757
GATCTTT34950.020.4327166
AGTGTAG16850.020.07189469
ATAGTGT1409.322654E-619.72078169
ACACTAT7050.019.57326338
TAGACTC2655.456968E-1219.53187261
CTATATA9900.019.51156641
TCTTTTT29150.019.4092258
GATCTGG39250.019.336866
CTTTTTT29100.019.0869189
CGATCTG140600.018.917865
Produced by FastQC (version 0.11.2)