FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n02_zt2wtlarvs30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n02_zt2wtlarvs30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1181331
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG108480.9182862381500189No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29630.2508187798339331No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAATGGCGTAACGATGGCCACACTGTCTC14800.12528241449686836No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTCGC400.002595448934.5024820
TAGGTGT11200.028.6530269
GTATAAG756.157084E-527.604321
ACACTAT2300.026.99850738
ATCTGTG4400.026.6564887
GATCTTG8150.025.8195676
CGATCTG65100.024.8534665
CACTATA2400.024.4361539
ATCTGGC8100.024.275417
TAGTACT1301.848457E-723.8832974
GATCTGG19450.023.766326
TAGGATG750.001993694322.998735
ACAGTAT1052.2818223E-522.99872838
TTAGGTG14150.022.67461868
TAAGTTC11450.022.59509330
GTCTAGG2155.456968E-1222.4686341
AGTTCCG11350.022.18820232
GAGTTAA1403.7779137E-722.17734715
TGCGAGT1403.781006E-722.17546812
ATCTGGG7500.022.0778457
Produced by FastQC (version 0.11.2)