FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n02_zt2wtlarvs29.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n02_zt2wtlarvs29.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences718374
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG86421.2029945404482902No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12740.17734494845303422No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8870.1234732882871596No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7920.11024897894411548No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7850.10927455615041748No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGTGT2900.030.94230869
TTTCGGA2250.029.1398931
ACTGTTG605.4086494E-428.76047969
ATCTGGC4600.027.7485167
AGAGTTG500.007732406327.6004475
GATCTGG12700.026.8922626
TCTGGCC2150.025.6730448
TTGTGTA1901.8189894E-1225.4303269
ACACTAT4000.024.14702638
TCTGAGC2300.023.9987168
TGTTCCG1602.1991582E-923.7191335
TTAGGGA2950.023.390213
TAGTGGG750.001992917922.998777
CTGTTCC2005.2750693E-1122.4253644
GACACTA4650.022.25532337
CACTATA4350.022.20416339
TAGATAC2650.022.1324335
GGGACAC8300.021.61179735
AGTCCGT800.002899283821.5613466
ATGTAGG1455.275815E-721.4186131
Produced by FastQC (version 0.11.2)