FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n02_zt2wtlarvs28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n02_zt2wtlarvs28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4727778
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG240680.5090763568001713No Hit
GTATAGATTCTGTTTGACGACCCGCTGAATTTAAGCATATTACTAAGCGGAGGAAAAGAAATCAACTGAGATTCC61950.1310340713967534No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA48050.10163336772581115No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATTC23150.030.9947594
TCTGGTC12550.028.8622848
GATCTGG66450.028.553076
ACGACCC25300.027.13280717
CAATACT25200.026.8306674
GACGACC26850.025.95190416
ATCTGGC25000.025.8039827
CGACCCG27350.025.60900718
TGGTCCG15000.025.52800410
CGATCTG232200.025.4495585
CTGTTTG27950.025.17870910
GACCCGC28600.024.61037419
ACCCGCT28750.024.36376820
GTGCAAC27850.024.15430511
TCAATAC28250.023.5688153
TCTGAGC9750.023.352028
CGTGCGA11550.023.29687910
CCGATCT624350.023.1229524
CCGCTGA31000.022.9293522
CGCTGAA30650.022.8534523
Produced by FastQC (version 0.11.2)