FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n02_zt2wtlarvs27.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n02_zt2wtlarvs27.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1429175
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG162311.1356901709027936No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA111490.7801004075777984No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21200.14833732747914005No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15620.10929382335963056No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14590.10208686829814403No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTATA5700.026.02301639
TCACGTT700.001331727124.65008469
GATTCGA700.001334174724.642327
TTTCGGA3600.023.9653631
ACACTAT6200.023.92438538
GACACTA6350.023.90331837
CGTGTAG7200.023.00674669
TAAGGGT1502.8878276E-823.0003034
GTATAGT1605.7030775E-821.5688251
ACTATAT6100.021.48757640
TACGTGT14150.021.46212269
GATCTTT31900.020.656296
ATCTTTT27600.020.624557
TAGTATA850.00412536420.29012344
GTACTAA1409.322335E-619.7200681
CTAGGCC1409.347741E-619.7138569
ATCTGAG8150.019.4719687
CGATCTG113500.019.1203045
GGACCGT6700.019.0518236
TCTTTTT30350.018.97912613
Produced by FastQC (version 0.11.2)