FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n02_zt2wtlarvs25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n02_zt2wtlarvs25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1069564
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG159791.4939732451727994No Hit
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAATCAAGAACGAAAGTTAGAGGTTCGAA19190.17941890340362987No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTT15590.14576032850769097No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTA12500.1168700517220101No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGATCTGAAAACCAATGAAAGCAGAACAG11250.10518304654980908No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATG10960.10247166134985845No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTAGG652.3187487E-531.8514211
ATCTGGG9600.025.5162667
CACTATA1801.2732926E-1124.91496339
GAGGTGT18450.023.9389269
TTTCGGA2750.023.8403051
ACACTAT1902.7284841E-1123.60364738
CGATCTG57250.023.5630345
TAAGGTA4450.023.259015
GATCTGG20850.023.166056
TATAGTG750.001992640823.0005785
ACCGGTA3750.022.99842615
CCGATCT132850.022.2291014
GGGACGT7650.022.09652716
ATATAAG3750.022.0795212
TTCCGAT136100.021.8250242
TAATGTG1906.7484507E-1021.7900225
GGACGTA7550.021.4753517
CATGTAA2251.0913936E-1121.46720526
TCCGATC137950.021.43233
TATAAGA3900.021.2303093
Produced by FastQC (version 0.11.2)