FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n02_zt2wtlarvs24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n02_zt2wtlarvs24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences652799
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG140162.1470621125338734No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17790.27251879981433796No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGGG1503.45608E-1127.6010442
TATGGCG500.007731082427.6010425
ATATATC756.164339E-527.59681337
TCTAGGG1653.6379788E-1227.1828462
TTTCGGA1800.026.8384571
AGAGTGT6750.025.04922969
CTCTAGG700.00133174624.6475641
CTGTCTT750.001990779823.0026328
TTACCAT750.00199166723.0008710
CAAAATA4400.022.7412453
TCCTATG2450.022.5314642
GTGCTAC2057.2759576E-1121.88222956
AATTCTT953.0597017E-421.79363822
TCTATGC953.0628743E-421.7902978
TGCGATA1756.202754E-921.6815544
GCATTTG1154.591953E-521.00401358
ACAAAAT4800.020.84613452
TTAAGCG1004.3286948E-420.7023748
GTAACTA1004.3286948E-420.7023753
CTATATA2700.020.44051741
Produced by FastQC (version 0.11.2)