FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n02_zt2wtlarvs23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n02_zt2wtlarvs23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences636310
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG97711.535572283949647No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19200.30173971806195093No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGC7600.11943863839952225No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGGA1250.038.6661
ATAACCT2600.033.17973357
TATCCTA605.42063E-428.7489855
TGCGACA854.430696E-628.40629463
CACTATA2700.028.11232839
GCCCTAA500.007707019327.6185721
CCAATTT500.007721829727.6077169
TGTACGG500.007733694327.5990245
CTGTACT906.9016605E-626.83238428
AGGATAT2100.026.28685434
ACACTAT3550.026.24057438
TCTGTAC1359.41327E-925.55465327
GACACTA4200.025.4653937
ACTATAT3400.025.36276240
TAGAATC1101.149414E-625.09594261
GACTGTA1101.150991E-625.09199737
TAGCTCC700.001333466424.6419854
AACTCAC3800.024.51807654
GGATATA1551.5206751E-924.48493235
AATAACC3300.024.05027856
Produced by FastQC (version 0.11.2)