FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n02_zt2wtlarvs22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n02_zt2wtlarvs22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences822345
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG88341.0742449944974433No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31540.3835373231429631No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTAT2250.032.1990338
GTCTAGT450.004618486430.6713451
TTTCGGA2400.030.1921061
CACTATA2050.028.60889639
AGTTCCG4800.026.59133732
GCTAGAC658.6503563E-426.54089453
GACACTA4150.025.77031137
TAACGAC3900.025.6593255
GGTAAGT4950.025.09168428
TAAGTTC4950.025.0886330
CTTGTCG5100.025.03170821
ACTTATG1401.38261385E-824.64961669
GTTATTA851.4413186E-424.3566571
GGGTAAG5100.024.35369327
AAGTTCC5600.024.02460331
ATCTGGC6400.023.1775827
ACGACTT4050.023.00490857
TATTAGG1052.280284E-522.9993112
GGGACAC6350.022.81821335
TGCACGA5000.022.76793342
Produced by FastQC (version 0.11.2)