FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n02_zt2wtlarvs21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n02_zt2wtlarvs21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2573777
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG260521.0122089054335321No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA98150.3813461694622339No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGGA6700.030.3859481
CTTGTGT26250.024.58529569
GATCTGG54700.024.2801176
CACTATA6700.024.2005839
CGATCTG193100.022.7774585
ATCTGGG20200.022.3716147
TCTGAGC8400.021.3550918
ATCTGAG15050.021.3168897
ATCTGTG14550.020.3897957
ATCTGGC22050.020.3381827
CGATCTT96050.020.2921685
ACACTAT8000.020.26798438
ATACTGT5800.020.22376667
CCGATCT540200.019.9300384
ACTATAT9950.019.76153840
TTCCGAT556500.019.5085872
ACAGTAT3950.019.21446438
ATCTTTT30550.019.196197
TCCGATC564150.019.1951273
CCTTGTG34150.018.89130868
Produced by FastQC (version 0.11.2)