FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n02_zt2wtlarvs20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n02_zt2wtlarvs20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2154011
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG211450.9816570110366195No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA61800.2869066128260255No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGGA8050.030.4358771
CGATCTG147100.025.0467155
GATCTGG46850.024.5203596
GATCTTG17800.023.6446026
ATCTGGG17900.023.5125127
AGTGTGT31850.023.18717669
ATCTGCG13050.022.9985777
CCGATCT435900.021.5255414
TTCCGAT440800.021.3699762
GATCTAG24900.021.1974836
TCCGATC448700.021.0004433
GATCTGC63400.020.3504736
TGTGTAG19450.020.22687369
TCTGCGC9650.020.0194858
CGATCTA85500.020.0127965
ATCTGAG11900.020.0029647
GTATTAT1751.4705074E-719.7190171
ATCTGGC20550.019.6411197
GATCTTT38650.019.6365596
CTTCCGA489150.019.4640391
Produced by FastQC (version 0.11.2)