FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n02_zt2wtlarvs1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n02_zt2wtlarvs1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80066
Sequences flagged as poor quality0
Sequence length75
%GC66

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1770622.114255739015313No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATAAT150.002350059868.9812645
TCACAGT150.002350059868.9812613
TAGCGCG150.002350059868.981264
TGAAAAC150.002350059868.9812666
AGTTGGT150.002350059868.9812617
CACAGTT150.002350059868.9812614
GGCATTT150.002350059868.9812632
ACGGGGA252.5561667E-455.18501328
AGACCGT252.5561667E-455.1850138
AACGACG200.00730583251.8006623
AACAGAT200.00730583251.8006660
GCCAAGT200.00730583251.8006619
ACAGTTG200.007342062451.7359515
AACTGAA200.007342062451.7359550
TCTTTAT200.007342062451.735953
TTGTTGG200.007342062451.7359530
AAGATAA200.007342062451.7359544
GACGAGT200.007342062451.735954
TTACCTG200.007342062451.7359566
TAATGAC200.007342062451.7359548
Produced by FastQC (version 0.11.2)