FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n02_zt2wtlarvs19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n02_zt2wtlarvs19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences711547
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20390.2865587234574807No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12000.16864662488914997No Hit
TTCCATCACTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCACCCACTTAGA7190.1010474360794157No Hit
CTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCACCCACTTAGAGCTGCATT7170.10076635837126711No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGGT901.9968866E-730.6605133
CGTGTAG2350.029.36819369
GTATTAT1650.029.2750841
CGCAAAT906.8894224E-626.83926669
CACTATA1057.685394E-726.28228639
TACGTGT5350.025.15509469
TCTGCGC1701.6734703E-1024.353198
GATCTGG8700.023.396796
CGATCTG27050.023.340355
ATCTGGG5050.023.2279597
GAGAGTA750.001991347223.00185225
CGAATCT902.1240878E-423.00185228
TCTAGGG1202.4569345E-622.9986172
CCGATCT63850.022.7973024
ATTATAG2005.2750693E-1122.4205023
GTATAGT2800.022.1803551
TCCATCA3900.022.1140562
TCCGATC66800.022.0487173
TTCCGAT66500.022.0475462
GCGCAAA1103.26617E-521.95322468
Produced by FastQC (version 0.11.2)