FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n02_zt2wtlarvs16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n02_zt2wtlarvs16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1202242
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG393083.2695580423908No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17030.141652013488133No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGC12310.1023920308889558No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATTA602.9129842E-740.246027
TTTCGGA3600.030.6725671
AGATTAC906.911767E-626.8306838
AGGGATT1457.039489E-1026.16982515
CACTATA2800.025.87459639
GACGTTA700.001334003424.64247139
AGTGTAG5750.024.60575769
AGAGTGT13050.024.3275369
GGATTCT2650.023.43164617
ATCTGTG3850.023.2963987
GATCTGG20100.022.1396036
ATCTGGG7100.021.8640377
TAAGTTC2550.021.6467230
ACACTAT3550.021.37994838
GGTCCAA5400.021.0812511
AGCATAC1004.334205E-420.7005372
TATTCGC1004.334205E-420.7005372
TACTAGT1004.334205E-420.7005372
GTCCTAG4600.020.2538951
CTCGCGT5650.020.15096529
Produced by FastQC (version 0.11.2)