FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n02_zt2wtlarvs15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n02_zt2wtlarvs15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences894459
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG133791.49576447886376No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31020.3468018098090578No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGGA2400.031.6343561
TGTCTTG3050.028.28072729
TCGCTAA500.00773973627.5958213
CACTATA4950.025.78685639
CTGTCTT3650.025.52238828
ACAATTT4100.025.2499669
GACACTA6350.024.99113337
CTATGGT851.4433391E-424.3519734
CTGAGTG851.4433391E-424.3519739
ACTATAC1151.696706E-624.0003873
GTCTTGG4250.023.5481411
ACACTAT6250.023.18307938
TGTGCCG902.1264127E-422.99908810
TCAGCGT2103.6379788E-1222.99908635
ACGGTGT7100.022.84350669
ACTATAT5750.022.79782140
TGCTCCC2155.456968E-1222.46422414
GTCTAGG2800.022.1851331
CGCTCTA1403.7732025E-722.1789368
AGCGTCA2207.2759576E-1221.95367437
Produced by FastQC (version 0.11.2)