FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n02_zt2wtlarvs14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n02_zt2wtlarvs14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1065896
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG55490.520594879800656No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30310.28436170132921035No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGGA4150.033.258311
CACTATA3400.031.45350639
ACACTAT3700.028.90322538
CTAGGCG809.566099E-525.8730479
GATCTGG25200.024.9147856
GTATATG1602.1973392E-923.7225251
ACTATAT4700.023.48538240
TCTGCGT2300.022.4983028
ATCTGGC9800.021.8248847
GACACTA6800.021.8145337
ATCTGGG10300.021.7702037
CTATATA5550.021.75307341
GTTCATA1154.6032394E-520.99940162
TAGGGGT4950.020.9075134
CGATCTG97550.020.8646475
ATCTAGC6350.020.6441127
TCTGGCC4900.020.4168268
GGGACAC8700.020.2226135
CGAAACT3250.020.16960128
CTCTAGG1551.0027925E-620.0354481
Produced by FastQC (version 0.11.2)