FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n02_zt2wtlarvs12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n02_zt2wtlarvs12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1754435
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA95240.5428528272634779No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG81410.46402403052834673No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG2550.029.7713871
TTTCGGA7200.028.2771781
CACTATA4700.024.95735439
ACACTAT4800.024.43740738
CTGTTAG1151.6951835E-624.0053861
GGTCCAA3750.022.99925811
ATCTTTT31700.022.19991112
GATCTGG33550.021.5933236
CGATCTG120600.020.4527385
ATCTGGC14250.020.3356067
TAAGTTC6450.020.32260930
GTCTTAC5550.019.8963571
AGTGTGT24000.019.84081869
TGTGTAG14850.019.7507869
TCCTAGA4900.019.7153362
CTTTTTT34300.019.71421214
TCTAGGG4250.019.4833932
TCTTTTT35100.019.26323913
GATCTTT39400.018.73797411
TCGTAAT3500.018.728535
Produced by FastQC (version 0.11.2)