FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n02_zt2wtlarvs11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n02_zt2wtlarvs11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1687608
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG120470.7138506098572655No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA57070.3381709496518149No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGGA3650.030.2574441
ACACTAT6550.029.49453738
TAGTCCG1551.8189894E-1228.93558310
CACTATA6950.026.80425839
GACACTA7850.026.36795437
CGTGTAG9650.025.39178769
ACTATAT7650.024.79665440
TCTAGGG4150.024.1142352
CTGTGCG1151.6988197E-623.999379
CTAGTCC1752.419256E-1023.6565239
TGCGCTA7700.023.29878210
GCGCTAT7800.023.00007811
CGCTATC7800.022.99530612
GTACTGG55000.022.840931
GCCTAGG2155.456968E-1222.4731881
AGTCCGG2005.2750693E-1122.42507711
GATCTGG29650.022.1080026
GCTATCG7900.021.83228113
CTCTAGG1605.7007128E-821.5702481
TACGTGT18150.021.29627469
Produced by FastQC (version 0.11.2)