FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n02_zt2wtlarvs10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n02_zt2wtlarvs10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1392727
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26590.19092040292174992No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14320.10281986347647457No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGGCC4550.025.019278
ATCTGTG5450.024.68547
TATTATG1602.2009772E-923.7212922
CGATCTG77850.023.708195
ATCTGGC9300.022.6265837
GATCTGG23800.022.6109966
ATAGTGT10100.021.52984469
TAGATTG5000.021.38922119
GACACTA3750.021.1592337
GGGAACG8500.021.10359616
AAGTTCC17150.020.71489731
CTCTAGG2501.8189894E-1220.7081721
CTGTGCG1004.3386084E-420.6977589
ATCTAGC6850.020.64747
CTATATA4350.020.61401641
TCTAGGG2553.6379788E-1220.2962952
GTAAGTT17900.020.23814829
TAAGTTC17900.020.04108430
TAGGACA1551.0054773E-620.030814
TTTACCG6200.020.0286530
Produced by FastQC (version 0.11.2)