FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n01_zt2wtlarvs9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n01_zt2wtlarvs9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146793
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG3093321.07253070650508TruSeq Adapter, Index 11 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG129568.826033938948044TruSeq Adapter, Index 11 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGG77465.276818376898081TruSeq Adapter, Index 11 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG56153.8251142765663215RNA PCR Primer, Index 11 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGG35082.389759729687383TruSeq Adapter, Index 11 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGG18141.2357537484757448TruSeq Adapter, Index 11 (96% over 28bp)
TATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGG14320.9755233560183387RNA PCR Primer, Index 11 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGGGG12790.8712949527566028RNA PCR Primer, Index 11 (96% over 28bp)
CACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGG8730.5947150068463756TruSeq Adapter, Index 11 (96% over 28bp)
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGG5390.36718372129461213TruSeq Adapter, Index 11 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG4320.29429196215078374Illumina Single End Adapter 1 (96% over 27bp)
TTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGG4320.29429196215078374TruSeq Adapter, Index 11 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGGGG4310.2936107307569162TruSeq Adapter, Index 11 (96% over 28bp)
TCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGG4080.27794240869796244TruSeq Adapter, Index 11 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAGGG3770.2568242354880682TruSeq Adapter, Index 11 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG3710.2527368471248629Illumina PCR Primer Index 9 (96% over 26bp)
TATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGG2840.1934697158583856RNA PCR Primer, Index 11 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAGGGGGG2400.1634955345282132TruSeq Adapter, Index 11 (96% over 28bp)
CTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG2250.15327706362019988RNA PCR Primer, Index 11 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGCTCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG2200.1498709066508621Illumina PCR Primer Index 9 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG2000.136246278773511Illumina PCR Primer Index 9 (96% over 26bp)
ATACACATCTCCGAGCCCACGAGACCGTACTAGATGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG1940.13215889041030565Illumina PCR Primer Index 9 (96% over 26bp)
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGGGGGGG1850.12602780786549767TruSeq Adapter, Index 11 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACCGTACTAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG1720.11717179974521945Illumina Single End Adapter 1 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGAGGG1690.11512810556361679TruSeq Adapter, Index 11 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGG1620.11035948580654391Illumina PCR Primer Index 9 (96% over 26bp)
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTG1590.10831579162494125TruSeq Adapter, Index 11 (96% over 28bp)
GCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGG1560.10627209744333858RNA PCR Primer, Index 11 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGTGC150.002351490368.9996648
GTTCTGT150.002351490368.99966467
AAGCAGT150.002351490368.9996646
GTATCGA150.002351490368.9996646
CATATAC309.068503E-657.5193141
TGCTAGA252.5598521E-455.1997355
GGGGATC200.007346459751.74974411
CTAAGCC200.007346459751.74974427
ACTAGGA200.007346459751.74974429
TTGGTGT200.007346459751.74974422
GTACTAA200.007346459751.74974430
ATACGCT200.007346459751.74974463
GCTCCGC200.007346459751.74974411
GTAAACT200.007346459751.7497443
ATATACA352.263179E-549.2854732
GCTAGAA352.263179E-549.28547356
AGTACTA352.263179E-549.28547329
CTTATAC65150.046.9866181
ATGATCA306.2935374E-445.9997759
TTATACA67950.045.03512
Produced by FastQC (version 0.11.2)