H32MNAFXY_n01_zt2wtlarvs96.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H32MNAFXY_n01_zt2wtlarvs96.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9568
Sequences flagged as poor quality	0
Sequence length	75
%GC	50

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG	1178	12.311872909698996	TruSeq Adapter, Index 1 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG	779	8.141722408026755	TruSeq Adapter, Index 1 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGG	518	5.4138795986622075	TruSeq Adapter, Index 1 (96% over 29bp)
ACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG	314	3.281772575250836	RNA PCR Primer, Index 1 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGG	304	3.177257525083612	TruSeq Adapter, Index 1 (96% over 29bp)
TTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGG	132	1.3795986622073577	RNA PCR Primer, Index 40 (96% over 30bp)
ACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGGGG	119	1.2437290969899666	RNA PCR Primer, Index 1 (96% over 29bp)
TATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGG	101	1.055602006688963	RNA PCR Primer, Index 1 (96% over 29bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	95	0.9928929765886287	No Hit
CACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGG	73	0.7629598662207357	RNA PCR Primer, Index 40 (96% over 30bp)
TTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGG	68	0.7107023411371237	RNA PCR Primer, Index 40 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG	37	0.3867056856187291	Illumina Single End Adapter 1 (100% over 25bp)
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTATGCTTGAAAAAAAAAAAGGGG	37	0.3867056856187291	Illumina Paired End PCR Primer 2 (96% over 25bp)
CATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGG	35	0.3658026755852843	TruSeq Adapter, Index 1 (96% over 29bp)
TATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGG	33	0.34489966555183943	RNA PCR Primer, Index 1 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAGGGGGG	26	0.2717391304347826	TruSeq Adapter, Index 1 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCGCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG	23	0.2403846153846154	Illumina Paired End PCR Primer 2 (96% over 25bp)
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGGGGGGG	22	0.229933110367893	TruSeq Adapter, Index 1 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCGCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGG	21	0.21948160535117056	Illumina Paired End PCR Primer 2 (96% over 25bp)
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGGGG	21	0.21948160535117056	TruSeq Adapter, Index 1 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCGCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG	20	0.20903010033444816	Illumina Paired End PCR Primer 2 (96% over 25bp)
ATACACATCTCCGAGCCCACGAGACATCTCAGGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG	18	0.18812709030100336	Illumina Single End Adapter 1 (100% over 25bp)
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG	17	0.17767558528428093	Illumina Paired End PCR Primer 2 (96% over 25bp)
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCGCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGG	17	0.17767558528428093	Illumina Paired End PCR Primer 2 (96% over 25bp)
CACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGGGGG	16	0.16722408026755853	RNA PCR Primer, Index 40 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTGTGCTTGAAAAAAAAAAAGGGG	14	0.1463210702341137	Illumina Paired End PCR Primer 2 (96% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	14	0.1463210702341137	No Hit
ACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGG	13	0.1358695652173913	RNA PCR Primer, Index 40 (96% over 30bp)
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTG	13	0.1358695652173913	TruSeq Adapter, Index 1 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTATGCTTGAAAAAAAAAAAGGGGGGG	12	0.1254180602006689	Illumina Paired End PCR Primer 2 (96% over 25bp)
ATACACATCTCCGAGCCCACGAGACATCTCAGGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGG	12	0.1254180602006689	Illumina Single End Adapter 1 (100% over 25bp)
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGAGGG	11	0.1149665551839465	TruSeq Adapter, Index 1 (96% over 29bp)
ACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGGGGGGGGG	11	0.1149665551839465	RNA PCR Primer, Index 1 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAGGG	11	0.1149665551839465	TruSeq Adapter, Index 1 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACATCTCAGGATGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG	10	0.10451505016722408	Illumina Paired End PCR Primer 2 (96% over 25bp)
GCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGG	10	0.10451505016722408	RNA PCR Primer, Index 1 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGG	10	0.10451505016722408	Illumina Single End Adapter 1 (100% over 25bp)
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGCTCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG	10	0.10451505016722408	Illumina Paired End PCR Primer 2 (96% over 25bp)
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTGTGCTTGAAAAAAAAAAGGGGG	10	0.10451505016722408	Illumina Paired End PCR Primer 2 (96% over 25bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCTTA	20	0.007138752	51.75	45
CTTATAC	280	0.0	49.285713	1
TTATACA	340	0.0	41.602943	2
TATACAC	350	0.0	40.414288	3
TCGCGTA	55	6.655402E-6	37.636364	35
GCGTATG	65	4.7368667E-7	37.153847	37
CGCGTAT	60	1.2089173E-5	34.500004	36
ATCGCGT	50	1.7272201E-4	34.5	34
GGGGGGG	520	0.0	32.509617	69
GGATCGC	55	3.023351E-4	31.363638	32
GATCGCG	55	3.023351E-4	31.363638	33
TTCTGCT	445	0.0	29.460672	52
TCTGCTT	450	0.0	29.133333	53
CTGCTTG	450	0.0	29.133333	54
ATCTCGT	380	0.0	29.052631	37
TCTCGTA	380	0.0	29.052631	38
CTTCTGC	440	0.0	29.011366	51
CTCGTAT	405	0.0	28.962961	39
TGCTTGA	465	0.0	28.935484	55
AGGATCG	60	5.0350727E-4	28.750002	31

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content