FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n01_zt2wtlarvs94.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n01_zt2wtlarvs94.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1448092
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG42360.29252285075810097TruSeq Adapter, Index 1 (96% over 29bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA42210.2914870049692975No Hit
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG21300.1470901020100933TruSeq Adapter, Index 1 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGG17740.12250602862249084TruSeq Adapter, Index 1 (96% over 29bp)
GTCCTGGAGACAGTGGGGAAGTCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTACCT15020.10372269165218784No Hit
GTATAGACCGTACCCGAAACCGACACAGGTGGTCAGGTAGAGTATACCAAGGCGCTTGAGAGAAGTGTCCTGAAG14540.10040798512801671No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT17100.031.67517347
GTATGCC18100.031.64061242
GTCTTCT16000.030.83411249
ATGCCGT18300.030.72924844
TGCCGTC17900.030.6449945
CTTGAAA18700.029.33397357
TTTCGGA5750.028.8136831
GCCGTCT19150.028.104246
CTTCTGC18150.027.18158751
TCGTATG20200.026.9849240
CTCGTAT19300.026.81324439
TCTTCTG19100.026.01024850
TATGCCG21950.025.9337243
GCTTGAA21250.025.81389856
TCTGCTT20250.025.3849753
TGAAAAA23500.024.6636259
TGCTTGA22200.024.55384455
TTCTGCT20900.024.43041452
GATCTTT42000.024.0676526
ATCTTTT39200.023.5865357
Produced by FastQC (version 0.11.2)