FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n01_zt2wtlarvs93.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n01_zt2wtlarvs93.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1781719
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA59730.3352380481995197No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG45130.2532947114556224TruSeq Adapter, Index 1 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG19980.11213889507829236TruSeq Adapter, Index 1 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGG19380.10877136069155688TruSeq Adapter, Index 1 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC19500.032.5533742
GTCTTCT18850.030.93058249
ATGCCGT20550.030.05065344
CTTGAAA20900.030.04262257
CCGTCTT20500.029.9556647
TGCCGTC21250.029.38545445
TATGCCG22100.029.1918843
CGCGTAT1800.028.7495839
TGCTTGA21600.028.4301455
GCTTGAA22100.027.9430356
CTCGTAT20300.027.87151139
GCCGTCT22650.027.264546
TCTTCTG21400.027.08371750
TTTCGGA6650.026.9861371
CGTATGC24100.026.33986341
ATCTTTT56000.025.7514087
CTGCTTG23200.025.13109854
CGTCTTC23200.024.98239348
TCTCGTA22000.024.46327838
GATCTTT63400.024.3781916
Produced by FastQC (version 0.11.2)