FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n01_zt2wtlarvs92.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n01_zt2wtlarvs92.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2389233
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA105270.4406016491484924No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG65310.27335132236998233TruSeq Adapter, Index 1 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG30160.12623297937036698TruSeq Adapter, Index 1 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGG25960.10865411619544849TruSeq Adapter, Index 1 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGGA9550.030.717381
TATGCCG30050.029.84990743
GTATGCC30550.029.5872242
ATGCCGT31000.028.9351544
TGCCGTC30150.028.60663645
CCGTCTT29800.028.36376647
CTTCTGC28500.027.47863251
CTTGAAA32400.027.15246457
GTCTTCT29300.027.081649
TCTCGTA30350.026.37201938
GCCGTCT32450.026.04746446
TCTTCTG31050.025.7774850
CGTCTTC32000.025.33564448
GCTTGAA34250.025.2829256
TTCTGCT31600.024.3462452
TCTGCTT32650.023.7746153
CGCGTAT2350.023.48909239
TTGAAAA39300.023.17530658
CTCGTAT35750.023.16057239
TGCTTGA37000.023.12405855
Produced by FastQC (version 0.11.2)