FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n01_zt2wtlarvs91.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n01_zt2wtlarvs91.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1003571
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA133361.3288546600091073No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39120.38980799564754265No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG35110.3498506832102562TruSeq Adapter, Index 1 (96% over 29bp)
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26960.26864068411701814No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25110.2502065125437064No Hit
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG16380.1632171515518085TruSeq Adapter, Index 1 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGG15200.1514591394131556TruSeq Adapter, Index 1 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAGGT350.001347631339.42923
CCGTCTT14450.035.3344447
CTAGGTT400.002595976534.5005463
GTATGCC15800.033.8437942
TGCCGTC15500.033.38596345
CTTCTGC13800.032.9988851
GCCGTCT15550.032.83489646
GTCTTCT14450.032.46948649
GCTTGAA15750.032.41794656
ATGCCGT16400.032.18488344
TATGCCG16650.032.11602843
CTCGTAT15750.031.54178639
CGTCTTC15150.031.42467748
TCGTATG16650.031.28722840
CTTGAAA16600.031.17364157
TTCTGCT14400.031.14477752
TGCTTGA16150.030.54695155
CGTATGC17550.030.46905141
TCTTCTG15300.030.2146650
TCTCGTA16300.029.84254538
Produced by FastQC (version 0.11.2)