FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n01_zt2wtlarvs90.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n01_zt2wtlarvs90.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1350967
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGACCGTACCCGAAACCGACACAGGTGGTCAGGTAGAGTATACCAAGGCGCTTGAGAGAAGTGTCCTGAAG16350.1210244217660387No Hit
GTCCTGGAGACAGTGGGGAAGTCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTACCT15880.11754543227184677No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15690.1161390322635564No Hit
GTCCTGAAGGAACTCGGCAAATTGCCTCCGTACCTTCGGAAGAAGGAGGCCCTCATTGTGCGCAAGCATTTTGAG13710.10148286375610953No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG39050.028.0939355
GATATAT500.00771833727.6124841
TTCCGAT109400.027.3111022
TCCGATC109350.027.292043
ATCTGAG3400.025.3669137
CCGATCT118900.025.1289714
ATCTGTG2900.024.9820357
ATCTGAC3250.024.4146797
GATCTGC13250.024.2143946
CTTCCGA126000.023.9691721
ATCTTTT18950.023.4848087
CGATCTA19400.023.2957155
GATCTGA11400.022.6967126
CTAGGAC3650.022.6859553
CTTTTTT19550.022.4119459
ATCTAGC3600.022.0410277
GATCTTT23000.021.8993646
ATCTGCT4100.021.8774157
TAGAACC953.0650717E-421.7904554
TCTTTTT19800.021.779678
Produced by FastQC (version 0.11.2)