H32MNAFXY_n01_zt2wtlarvs8.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H32MNAFXY_n01_zt2wtlarvs8.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2443555
Sequences flagged as poor quality	0
Sequence length	75
%GC	51

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG	8674	0.3549746169003767	RNA PCR Primer, Index 46 (96% over 28bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6940	0.28401243270562765	No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG	4438	0.18162063059763336	RNA PCR Primer, Index 46 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGG	4350	0.1780193202117407	RNA PCR Primer, Index 46 (96% over 28bp)
GGTAGGGATACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCTCAGTAACG	3859	0.1579256452177258	No Hit
CTCTACTTGTGCGCTATCGGTCTCCCGCCAATATTTAGCTTTAGATGGAATTTACCACCCATTTTGAGCTGCATT	2758	0.11286834141240938	No Hit
GAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCTCAGTAACGGCGAGTGAAGCGGCAA	2586	0.10582941656725549	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GTATGCC	4200	31.131563	42
TGCTTGA	3890	31.129877	55
CCGTCTT	3990	30.60845	47
GCTTGAA	4075	30.47858	56
CTACTTG	1355	30.298328	3
GCCGTCT	4025	30.170866	46
TCTACTT	1375	30.108528	2
CTCTACT	1400	30.073568	1
CGTATGC	4335	30.0029	41
CTGCTTG	3670	29.799782	54
TACTTGT	1380	29.749443	4
TCGTATG	4225	29.72252	40
CTCGTAT	4180	29.547293	39
CTTCTGC	3765	29.50603	51
TGCCGTC	4275	29.13279	45
GTCTTCT	3845	28.802391	49
TCTTCTG	3905	28.536541	50
ATGCCGT	4540	28.496162	44
CTTGAAA	4525	27.752535	57
TGCGCTA	1530	27.736479	10