FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n01_zt2wtlarvs88.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n01_zt2wtlarvs88.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1126062
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG825327.3292589573220654TruSeq Adapter, Index 8 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG500254.442472972180928TruSeq Adapter, Index 8 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGG439563.9035150817628157TruSeq Adapter, Index 8 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGG279742.4842326621447133TruSeq Adapter, Index 8 (96% over 29bp)
ACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG200611.7815182467750446RNA PCR Primer, Index 8 (96% over 29bp)
TTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGG95250.8458681671169083TruSeq Adapter, Index 8 (96% over 29bp)
ACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGGGG91440.812033440432232RNA PCR Primer, Index 8 (96% over 29bp)
TATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGG66250.5883335020629414RNA PCR Primer, Index 8 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTATGCTTGAAAAAAAAAAAGGGG49840.4426044036651623Illumina PCR Primer Index 8 (96% over 27bp)
CACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGG44860.39837948532141215TruSeq Adapter, Index 8 (96% over 29bp)
TTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGG44340.3937616223618237TruSeq Adapter, Index 8 (96% over 29bp)
TATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGG26900.23888560310178303RNA PCR Primer, Index 8 (96% over 29bp)
CATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGG22770.20220911459582153TruSeq Adapter, Index 8 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACGCTCATGAATCGCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG21420.1902204319122748Illumina PCR Primer Index 8 (96% over 27bp)
ATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTATGCTTGAAAAAAAAAAAGGGGGGG21290.18906596617237773Illumina PCR Primer Index 8 (96% over 27bp)
ATACACATCTCCGAGCCCACGAGACGCTCATGAATCGCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGG20730.18409288298512871Illumina PCR Primer Index 8 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTGTGCTTGAAAAAAAAAAAGGGG20560.18258319701757098Illumina PCR Primer Index 8 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCGCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG19960.1772548936026613Illumina PCR Primer Index 8 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGGGG18940.1681967777973149TruSeq Adapter, Index 8 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCGCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGG18830.16721992217124812Illumina PCR Primer Index 8 (96% over 27bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17110.15194545238184043No Hit
ATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGGGGGGG13610.1208636824615341TruSeq Adapter, Index 8 (96% over 29bp)
ACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGG11610.10310267107850189Illumina PCR Primer Index 2 (96% over 28bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC335250.045.6062281
GTATTAT5050.043.72226749
TTATACA358700.042.6863752
TATTATG4950.042.5146650
ATTATGC4300.041.72044451
TATACAC373850.041.113433
GTATTGT1800.040.24953549
TATGACG3850.039.42811643
GTATGAC3950.039.30334542
TTGTGCT14800.038.695552
TTAATAC901.2187229E-1038.334592
CTAGTAT2450.038.01996639
TCTAGTA2750.037.6359338
TACTATG557.3948504E-637.63592540
TCTTGTG13100.036.60644550
GTGCTTG16200.036.20328554
TGTGCTT16950.036.0261353
GAATCTA3650.035.91739335
TAGTATG3000.035.64958640
CGTATTA6500.035.56112748
Produced by FastQC (version 0.11.2)